CRISPR

Du får viden om

Du vil få en gennemgang af gene targeting før og efter ”CRISPR craze”-æraen 2013, samt en gennemgang af princippet for geneditering baseret på alternative metodikker som zinc finger nucleases (ZFNs) og transcription activator-like effector nuclease (TALENs) og meganucleaser.

Du lærer princippet for anvendelsen af CRISPR/Cas9 som gen-editeringsteknik, med særligt fokus på dets anvendelse i eukaryote celler og desuden en gennemgang af metoder til identifikation af Cas9-inducerede genom-ændringer.

Du vil også opnå ny viden om afledte CRISPR/Cas9-applikationer med særlig vægt på Base Editing. CRISPR-specificitet og sikkerhed, herunder om utilsigtet ‘on target og off target’-begrebet vil blive gennemgået. Ydermere vil du opnå indsigt i de bioteknologiske og terapeutiske anvendelsesmuligheder for CRISPR/Cas9 og Base Editing, med særligt fokus på CRIPSR/Cas9 og Base Editing-baseret genterapi.

Kompetencer og udbytte

Du vil få grundlæggende indsigt i princippet i CRISPR/Cas9 genediterings metodikken, dets mange forskellige anvendelsesmuligheder som akademisk gen-modificeringsredskab og de afledte translationelle aspekter for anvendelse af CRISPR i bioteknologiske- og terapeutiske sammenhænge. 

Introduction

On the CRISPR course, you will gain insight into the principle of CRISPR/Cas9 gene editing and gene editing without the formation of double-strand breaks via Base Editing. The course gives you a thorough insight into the many different applications of the technique as well as academic gene modification tools as well as the derived translational aspects in biotechnological and therapeutic contexts.

Experience with standard molecular biology, DNA and cell culture techniques is an advantage but not a prerequisite for participation. The course is for laboratory technicians, bioanalysts and academics, but others can also participate with advantage

The course will alternate between cases and assignments. For example, we will work with practical case-based exercises on gRNA design for your own project, ethical considerations in CRISPR, etc.

You gain knowledge about

You will get a review of gene targeting before and after the “CRISPR craze” era 2013, as well as a review of the principle of gene editing based on alternative methodologies such as zinc finger nucleases (ZFNs) and transcription activator-like effector nuclease (TALENs) and meganucleases.

You will learn the principle of using CRISPR/Cas9 as a gene-editing technique, with a particular focus on its use in eukaryotic cells and also a review of methods for identifying Cas9-induced genome changes.

You will also gain new knowledge about derivative CRISPR/Cas9 applications with special emphasis on Base Editing. CRISPR specificity and safety, including whether the unintentional ‘on target and off target’ concept will be reviewed. Furthermore, you will gain insight into the biotechnological and therapeutic applications of CRISPR/Cas9 and Base Editing, with a particular focus on CRIPSR/Cas9 and Base Editing-based gene therapy.

Competences and benefits

You will gain basic insight into the principle of the CRISPR/Cas9 gene editing methodology, its many different applications as an academic gene modification tool and the derived translational aspects for the use of CRISPR in biotechnological and therapeutic contexts.

Har du spørgsmål eller brug for hjælp?

Kaj Reiter

50762669

KARE@zealand.dk

Hos os har vi tit interne kurser. Men ved at komme ud på et uddannelsessted som Zealand, møder man folk med samme uddannelse og job som én selv, som måske ser tingene fra en anden vinkel, fordi vi ikke arbejder samme sted. Det er meget inspirerende.

Jane Kensø, laborant, Center for Diagnostik, DTU

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